Steps for preparation
- 70
ml each
of sesame oil
was measured into a cleaned and
dried conical flask and heated up to
70°c, 1.4 ml
of water was
added at the
70°c temperature after which
six (6) drops
of Hydrogen peroxide was added and stirred with a magnetic
stirrer for 1
hour
- This method
is called degumming of
oil. Lecithin was
then isolated by adding 20 ml of
acetone to the
mixture because lecithin is insoluble
in acetone
-
This was followed
by purification of
the extracted lecithin
with the use of column
chromatography .