Method of Application

200 g inoculant is mixed with 200 ml of rice kanji or 0.1% CMC added solution and make a slurry. The seed quantity of 10 kg is mixed with slurry and make uniform coating of the inoculant over the seeds followed by shade drying for 30 minutes. The shade dried seeds should be sown within 24 hours.

 

Liquid inoculum: For small quantity of seeds (up to 5 kgs quantity) the coating can be done in a plastic bag. For this purpose, a plastic bag having size (21” x 10”) or big size can be used. The bag should be filled with 2 kg or more of seeds. The bag should be closed in such a way to trap the airs as much as possible. The bag should be squeezed for 2 minutes or more until all the seed are uniformly wetted. Then bag is opened, inflated again and shaked gently. Stop shaking after each seeds gets a uniform layer of culture coating. The bag is opened and the seed is dried under the shade for 20-30 minutes. For large amount of seeds coating can be done in a bucket and inoculant can be mixed directly with hand. The seed treatment can be done with any of two or more bacteria. There is no side (antagonistic) effect. The important things that has to be kept in mind are that the seeds must be coated first with Rhizobium, Azotobacter or Azospirillum. When each seed gets a layer of above bacteria then PSM inoculant has to be coated as outer layer. This method will provide maximum number of each bacteria required for better results. Treatments of seed with any two bacteria will not provide maximum number of bacteria on individual seed.